Restriction enzyme mapping

Fig. 2. Partial restriction enzyme map of plasmid pAMD 101. The blackened segment shows A. bronchisepticus DNA of 1.2 kb...

The calibration of the E. coli genetic map in minutes was a temporary expedient. It was followed by physical maps expressed directly as micrometers of DNA length (total length 1.6 mm) or thousands of nucleotide units (kb). A physical map obtained by restriction enzyme mapping is shown in Fig. 26-4B. To obtain this map DNA fragments were prepared using specific restriction endonucleases (Section E, 1). 

Electrophoretic analyses of restriction enzyme digests and construction of a restriction enzyme map (see Experiment 15)... 

Blair, D. and McManus, D.P. (1 989) Restriction enzyme mapping of ribosomal DNA can distinguish between fasciolid (liver fluke) species. Molecular and Biochemical Parasitology 36, 201-208. 

Kaukas, A., Johnston, D.A., Kane, R.A. and Rollinson, D. (1994) Restriction enzyme mapping of ribosomal DNA of Schistosoma spindale and 5. leiperi (Digenea) and its application to interspecific differentiation. Systematic Parasitology 27, 13-1 7. 

Figure 22.4 Restriction enzyme map of a 21.5 kilobase (kb) DNA fragment from Pseudomonas strain ADP (adapted from de Souza et al., 1995).a...

Collections of tools for DNA and Protein sequence analysis, searching and presentation. Supports restriction enzyme mappings, open reading frames, primer design, sequence alignment, property calculations. 

Restriction enzyme mapping 5 -end labelling fragments Sequencing by Maxam -Gilbert method... 

At the DNA level, the isolated genes are dissimilar. For example, in wheat leaf and wheat endosperm, there is only 55.7% identity (Olive et al., 1989) and, on the basis of Southern blot hybridization analyses and restriction enzyme mapping, it is concluded that there are at least two distinct gene families in wheat. For spinach leaf and rice endosperm, there is only approximately a 50% identity (B. S. White and J. Preiss, unpublished results, 1998). 

Restriction enzyme map of a-globin gene cluster and some of the deletions (indicated as dark lines). 

Restriction enzyme map of Filipino-type deletion in ot-globin gene cluster. 

The chemical properties of the platinated DNA, termed M13-12A-Pt(-)-Stu I, were investigated by enzymatic, digestion and gel electrophoresis experiments. Platinum completely inhibits cleavage of the DNA by Stu I, as expected from the earlier restriction enzyme mapping studies. In addition, the cis-[Pt(NH3)2 d(pGpG) ] and cA-[Pt(NH3)2 d(pApG) ] intrastrand crosslinks were... 

You have just cloned an EcoRl fragment that is 1650 base pairs Obp) and contains the gene for the hormone leptin. Your first job is to prepare a restriction enzyme map of the recombinant plasmid. You know that you have cloned into a plasmid vector that is 805 bp and that has only one EcoRl site (the one into which you cloned). There are no other restriction enzyme sites in the plasmid. The following table shows the restriction enzymes used and the DNA fragment sizes that result. Draw a map of the circular recombinant plasmid and a representation of die gel from which the fragment sizes were obtained. 

One such plasmid is pBR322. The restriction enzyme map of pBR322 is illustrated in Figure 24.22. This plasmid has two antibiotic resistance genes one for ampicillin and one for tetracycline. Within the antibiotic resistance genes are several restriction enzyme sites that are convenient for cloning. 

Figure 2.7. Restriction enzyme maps of the repeating units of the mitochondrial genomes of several spontaneous petite mutants. The molecular weights of the repeat units are indicated, along with the positions of Hae III (A), Hpa II (T) and other restriction sites. In the case of a.n, five isolated Hpa II sites and Mine II site are not shown. The broken lines indicate corresponding restriction sites in dilferent repeat units. (From...

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