Restriction enzymes Dictyostelium

Kuspa A,. Loomis WF (1992) Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc Natl Acad Sci USA 89 8803-8807. 

Even though Man-6-P occurs in Dictyostelium, it is not known if it is used to target lysosomal enzymes [41,42] since no Man-6-P receptor has been identified. M. Lammertz has recently isolated a group of mutants that are partially (30-60%) deficient in GlcNAc-1-P transferase activity, and also have considerably reduced activities for several lysosomal enzymes [unpublished results]. The mutants show abnormalities in the early part of development, and they can only grow on bacteria, not in liquid medium. The restricted growth condition explains why such mutants were not detected previously. More detailed analysis should help to resolve whether Man-6-P is actually involved in lysosomal enzyme targeting. 

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