Restriction enzyme mediated integration,

Restriction enzyme-mediated integration (REMI) is a commonly used method for random mutagenesis that has been adapted for the use in D. discoideum by Kuspa and Loomis [40]. A plasmid containing an appropriate selection marker is Hn-earized with a restriction enzyme, and the linear plasmid is transformed by electroporation along with the restriction enzyme (Fig. 5.2). It is assumed that the restriction enzyme will cut certain chromosomal loci at specific recognition sites. If a transformed plasmid would Hgate with its sticky ends to the double-strand break introduced by the restriction enzyme, mutants can be isolated based on the selection marker present on the integrated REMI plasmid. 

Restriction endonucleases 168 Restriction enzyme-mediated integration (REMI) 667... 

Kuspa A,. Loomis WF (1992) Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc Natl Acad Sci USA 89 8803-8807. 

Tang J, Liu L, Hu S, Chen Y, Chen J (2009) Improved degradation of organophosphate dichlorvos by Trichoderma atroviride transformants generated by restriction enzyme-mediated integration (REMI). Bioresour Technol 100 480 83... 

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