Reproducibility pepsin digestion

Since digestibility trials are laborious and expensive to carry out, numerous attempts have been made to determine the digestibility of foods by reproducing in the laboratory the reactions that take place in the alimentary tract of the animal. Digestion in non-ruminants is not easily simulated in its entirety, but the digestibility of food protein may be determined from its susceptibility to attack in vitro by pepsin and hydrochloric acid. It is also possible to collect digestive tract secretions via cannulae and to use them to digest foods in vitro. 

Fig. 7 Identification of novel posttranslational modifications of histones H2A and H2B isolated from calf thymus nuclei by FT-ICR MS analysis of proteolytic peptides generated by the digestion of histone H2A with pepsin (a) or trypsin (b), and of histone H2B with trypsin (c) and V8DE (d). Reproduced from [375]...

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