Reporter gene expression quantification

Quantification ofLuciferase Reporter Gene Expression in Cell Lysate... 

Yu Y, Annala AJ, Barrio JR, Toyokuni T, Satyamurthy N, Namavari M, Cherry SR, Phelps ME, Herschman HR, Gambhir SS. Quantification of target gene expression by imaging reporter gene expression in living animals. Nat Med 2000 6 933-937. 

Yang, T.T., Sinai, P., Kitts, P.A. and Kain, S.R. (1997). Quantification of gene expression with a secreted alkaline phosphatase reporter system. Biotechniques, 23, 1110-1114. 

Tire complementary DNA of these aptamers was inserted in tandem into the untranslated region of an expression plasmid with a (f-galactosidase reporter gene. Chinese hamster ovary (CHO) cells were transfected with the expression plasmid and grown in the presence of H33258. Quantification of P-galactosidase activities 24 hours after transfection revealed that translation was prevented at concentrations of 5-10 mM of H33258 [80]. 

The first attempts to enable gene expression analysis by MS were reported only recently, when Smith and coworkers appHed the Invader assay (see Section 5.4.1.5 and Figure 5.12) to the detection and relative quantification of RNA [224]. In order to allow an inter-spectrum (and thus inter-sample) correlation. Invader assays of multiple target genes were multiplexed and a reference gene was included for normalization of the data. 

---