Repeated sequences sequence determination

The sequence of tectonic events in the Akfadou area is derived from an analysis of the tectonic subsidence of the basement surface. The sequence basically repeats the sequence determined for the Takhoukht area stretching through the Ordovician-Devonian, thermal activation during the Permian, and basement stretching in the Early Cretaceous, with thermal activation of the lithosphere from the beginning of the Cretaceous until the present. A high maturation level of organic matter is observed in the lower horizons of the sedimentary section = 1.00-1.19% at depths of 3.0 3.9 km. 

Sequencing The determination of the order in which the repeating units occur in a biopolymer, e.g. amino acids in a protein, sugar residues in a carbohydrate, etc. 

TES-32 is the most abundant single protein product secreted by the parasite. It is also heavily labelled by surface iodination of live larvae (Maizels et al., 1984, 1987), and is known by monoclonal antibody reactivity to be expressed in the cuticular matrix of the larval parasite (Page et al, 1992a). TES-32 was cloned by matching peptide sequence derived from gel-purified protein to an expressed sequence tag (EST) dataset of randomly selected clones from a larval cDNA library (Loukas et al., 1999). Because of the high level of expression of TES-32 mRNA, clones encoding this protein were repeatedly sequenced and deposited in the dataset (Tetteh et al., 1999). Full sequence determination showed a major domain with similarity to mammalian C-type (calcium-dependent) lectins (C-TLs), together with shorter N-terminal tracts rich in cysteine and threonine residues. Native TES-32 was then shown to bind to immobilized monosaccharides in a calcium-dependent manner (Loukas et al., 1999). 

If an AED is available, check the rhythm to determine if defibrillation is advised. If so, then deliver one shock with the immediate resumption of chest compressions/rescue breaths. After five cycles, reevaluate the rhythm to determine the need for further defibrillation. Repeat this sequence of actions until helps arrives or the rhythm is no longer shockable. ... 

Fig. 8. Organization of the kringle IV repeats in apo(a). It has been determined that kringle IV repeats, which vary in amino acid sequence from the identically repeated kringle sequence (kringle IV types 1 and 3-10), are highly variable in the population, ranging from less than 10 to greater than 50. The numbers in brackets below the boxes refer to the kringle nomenclature described in McLean et at. (M24). Sequences corresponding to apo(a) kringle V and protease domains are indicated by stippled and solid boxes, respectively.

This set of equations is solved at any particular pressure by assuming a trial value of Ts and then in sequence, determining n and Tn from Figure 8, r from Equation 5, T2 from Equation 4a, 7 from Equation 2b, r from Equation 3b, and Ts from Equation 5. If the finally calculated value of Ts does not correspond to the initially assumed value, the procedure is repeated with another trial value of Ts until they do agree. 

PCR uses DNA polymerase to repetitively amplify targeted portions of DNA. Each cycle of amplification doubles the amount of DNA in the sample, leading to an exponential increase in DNA with repeated cycles of amplification. The amplified DNA sequence can then be analyzed by gel electrophoresis, Southern hybridization, or direct sequence determination. 

Tetraplex structures have also been observed for G-rich repeating sequences associated with the human fragile X syndrome 275/276 This is the most common cause of inherited mental retardation and appears to arise as a result of the presence of an excessive number of repeats of the trinucleotide sequence (CGG)n. For normal persons n = 60 or less for healthy carriers n may be as high as 200 but for sick individuals it may be much higher.275 The structure in solution, as determined by NMR spectroscopy, is shown in Fig. 5-26. Another variant of four-stranded DNA, which arises from cytosine-rich DNA, contains C CH+ pairs such as the following at low pH.277 279... 

Fig. 1. Schematic representation of the three distinct regions of LPS. The repating unit structure of the O-polysaccharide may be recognized by antibodies with specificities for determinants associated with the terminal non-reducing residues (antibody type 1 b) or internal sequences often spanning more than one repeating sequence (cf. Refs. [72, 73])...

---