Reoviruses binding sites

Bisaillon, M., and Lemay, G. (1997). Molecular dissection of the reovirus ill protein nucleic acids binding site. Virus Res. 51, 231-237. 

These three unique binding sites represent the second example of nonequivalence in the reovirus core structure. While site iii is partially similar to site ii, sites i and ii are entirely different both in terms of the secondary structure and the pattern of charged/hydrophobic/polar residues that the XI surface presents to g2. a2-i lies over the middle of a XI-A molecule, and a2-ii bridges from the middle of a Xl-B across to the carboxy-terminal part of a Xl-B from another decamer. a2-iii lies on the XI shell directly on an icosahedral 2-fold axis in one of two equally-likely, two-fold related orientations. Consequently, a2-iii has not been built into the 3.6A electron density maps, and instead a a2-ii model has been docked onto that site. It is clear, however, that the various versions of a2 differ only at the interface with the XI surface. The differences between a2-i and ii are subtle, and the most drastic change is an unravelled helix (residues 39-46) in cj2-ii with respect to a2-i. 

Kozak, M., 1982a, Analysis of ribosome binding sites from the si message of reovirus. Initiation at the first and second AUG codons, J. Mol. Biol. 156 807. 

Kozak, M., 1982, Sequences of ribosome binding sites from the large size class of reovirus mRNA, J. Virol. 42 467. 

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