Release of Oligosaccharides from Glycoproteins

Patel, T. P. and Parekh, R. B. Release of oligosaccharides from glycoproteins by hydrazinolysis. Methods Enzymol. 230 57—66, 1994. 

Profile Analysis of Oligosaccharides from Glycoproteins by PMP Labeling. Comparison of Chemical and Enzymatic Release Methods Using RP-HPLC and Mass Spectrometry... 

Thus, the effects of glycosylation inhibitors on intact cells may also be studied best with virus-infected cells. Before release of virus, the glycoproteins are detected in the water-insoluble, membranous fraction. Furthermore, the lipid-linked oligosaccharides may be rather specifically extracted from whole cells, and monosaccharide-lipids may also be determined.3-116 It is thus seen that the various tools of virology and of lipid and carbohydrate biochemistry have proved productive in establishing the mode of action of inhibitors of lipid-depen-dent glycosylation of proteins. 

Patel T, Bruce J, Merry A, Bigge C, Wormald M, Jaques A, Parekh R (1993) Use of hydrazine to release in intact and unreduced form both N- and -linked oligosaccharides from glycoproteins. Biochemistry 32 679-693... 

The lack of a pan-specific or broad specificity O-glycanase makes chemical methods the most appropriate approach for the non-specific release of intact O-linked oligosaccharides from glycoproteins for site analysis.219 The most common chemical methods employed for the release of O-linked glycans are based on (3-elimination. The methods were originally developed to release the glycans for... 

Patel, T., et al. (1993). Use of Hydrazine to Release in Intact and Unreduced Form Both N- and O-linked Oligosaccharides from Glycoproteins, Biochemistry 32 679-693. 

Almost total release of sialic acids from glycoproteins or oligosaccharides can be achieved108,107 by heating the material in 0.05 M sul-... 

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