Regional intestinal perfusion techniques

Lennemas et al. have developed a method for measuring human effective permeability (H-Peff) using a regional intestinal perfusion technique. In this method, a perfusion apparatus consisting of a multichannel tube with two inflatable balloons (10 cm apart) is swallowed by the patient and eventually located in the proximal jejunum. Dilute solutions of the test drag are introduced at the inlet located at the center of the 10 cm section, and the loss of drag is determined from the concentration in the outlet intestinal perfusate. In such a fashion, the H-Peff for 22 carefully selected drug molecules has been determined and a theoretical model of H-Peff has been developed. " The small size of the published H-Peff database is most likely due to the expense of the human measurement. 

Tannergren C, Knutson T, Knutson L and Lennernas H (2003) The Effect of Ketoconazole on the In Vivo Intestinal Permeability of Fexofenadine Using a Regional Perfusion Technique. BrJ Clin Pharmacol 55 pp 182-190. 

Tannergreen, C., Knutson. T. Knutson, L., and Lennernas, H. (2003) The effect of ketoconazole on the in vivo intestinal permeability of fexofenadine using a regional perfusion technique. British Journal of Clinical Pharmacology, 55, 182-190. 

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