Refractive Index Detector RID

A comparative study of the analysis of aliphatic amines by GC-FID, GC-TSD and HPLC with refractive index detector (RID), using isopropylamine as internal standard, gave good results in all cases. Determination of trimethylamine oxide by HPLC with a pulsed amperometric detector was problematic136. 

Hopia AI, Ollilainen VM. Comparison of the evaporative light scattering detector (ELSD) and refractive index detector (RID) in lipid analysis. J Liq Chromatogr 1993 16 2469-2482. 

Refractive index detector (RID) 1 x 10 7g 1 x 104 Universal, dependent on refractive index difference with mobile phase Relatively insensitive to flow fluctuations, but sensitive to temperature fluctuations nondestructive, cannot be used with gradient elution solvents must be degassed to avoid bubble formations laser-based Rl detectors offer higher sensitivity... 

Molecular weights were obtained by GPC in dichloromethane (1 mL/min) using a Waters pump model 6000, an injector (Rheodyne) and a refractive index detector (RID-6A Shimadzu), equipped with a PL gel 5)iim mixed-C linear column. The system was calibrated using polystyrene standards with low polydispersity. 

The water-insoluble portion was then washed with 10 mL of MeOH to separate MeOH-soluble and MeOH-insoluble portions. The obtained water-soluble and MeOH-soluble portions, the sum of which nearly corresponds to the supercritical water-soluble portion, were then analyzed by the high perfonnance liquid chromatograph (HPLC) (Shimadzu LC-lOA) which consists of a high pressure pump (Shimadzu Co., Model LC-IOAT). HPLC analysis conditions were as follows [Column SHI ODS-11, Column temperature 40 6. Carrier solvent CHjOH/HjO = 20/80 (O- -IO min), 20/80 - 100/0 (10 20 min), 100/0 (20- 30 mm), Flow rate 0.7 ml/min. Detector a spectrophotometric detector (SPD) (A =254 nm) or a refractive index detector (RID)], or [Column ULTRONPS-80P, Column temperature SOTl. Carrier solvent H2O, Flow rate 1,0 ml/min, Detector SPD (A =254 nm) or RID], To obtain the yields of monosaccharides and their decomposed products, their standard samples (Nacalai tesque, extra pure reagent) with known concentrations in water were analyzed by HPLC as a standard in a similar manner. 

The most commonly used analytical technique for sugars is HPLC with a refractive index detector (RID). Although the HPLC-RID method is simple, the RID lacks sensitivity and selectivity. Therefore, UV and fluorescence detection is frequently used, coupled with pre- or postcolumn derivatization, for analysis with higher sensitivity. Liquid chromatography-mass spectrometry (LC-MS) using electrospray ionization also requires pre- or postcolumn derivatization. LC-MS using atmospheric pressure chemical ionization does... 

The methods evaluated for KHI analyses are methyl orange/colorimetric, iodine complexation, and size exclusion chromatography (SEC). The parameters investigated in evaluating the colorimetric method are the matrix effects and interferences from sulfide and organics present. The preliminary results for SEC determination of KHI are presented, and the limitations of Corona Aerosol Detector (CAD) and refractive index detector (RID) are discussed in terms of linearity and selectivity. 

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