Reference Sequence project

Pruitt, K. D., T. Tatusova, and D. R. Maglott. 2003. NCBI Reference Sequence project Update and current status. Nucleic Acids Res 31 34-7. 

The next section deals with RefSeq information. RefSeq is short for the NCBI Reference Sequence Project, which is an effort aimed at providing a stable, reference sequence for all of the molecules corresponding to the central dogma of biology. The intention is that these reference sequences will provide a framework for making... 

Fig. 6.1. Summary data for the large-scale expressed sequence tag (EST) sequencing projects for Schistosoma mansoni and S. japonicum. Numbers refer to ESTs obtained for each of the six stages of the parasite s life cycle.

The spectacular success of gene sequencing projects is largely due to the application of automated sequencing methods using laser-induced fluorescence techniques. The automation refers to the read-out process the preparation and loading of... 

Genome Bioinformatics Site. University of California, Santa Cruz (UCSC). URL http //genome.ucsc.edu/. Contains the reference sequence and working draft assemblies for a large collection of genomes. Provides a portal to the Encyclopedia of DNA Elements (ENCODE) project. 

The NCBl handbook [Internet]. Bethesda (MD) National Library of Medicine (US), National Center for Biotechnology Information 2(X)2 Oct. Chapter 17, The Reference Sequence (RefSeq) Project. The Internet http //ncbi.nlm.nih.gov/ entrez/query.fcgi db=Books. 

The SWISS-PROT database [36] release 40.44 (February, 2003) contains over 120 000 sequences of proteins with more than 44 million amino adds abstracted from about 100 000 references. Besides sequence data, bibHographical references, and taxonomy data, there are highly valuable annotations of information (e.g., protein function), a minimal level of redundancy, and a high level of integration with other databases (EMBL, PDB, PIR, etc.). The database was initiated in 1987 by a partnership between the Department of Medicinal Biochemistry of the University of Geneva, Switzerland, and the EMBL. Now SWISS-PROT is driven as a joint project of the EMBL and the Swiss Institute of Bioinformatics (SIB). 

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