Recoverin, calcium binding

Recoverin, calcium binding in, 46 457 Recrystallization of actinide metals, 31 14 hot zone... 

DREAM is a calcium-binding protein that belongs to the recoverin EF-hand subfamily. One feature of the recoverin subfamily is the CPXG sequence at the beginning of the EF-1 motif This sequence abolishes the calcium-binding ability of EF-1. As a result, DREAM binds three calcium ions, two at EF-3 and EF-4 with higher affinities ( 5 pM) and one at EF-2 with lower affinity ( 50 pM). Compared to the other recoverins, DREAM has a long N-terminal sequence but loses the N-terminal myristoylation. No X-ray or NMR structure is available for DREAM at present, but the structural information is available for other recoverin members, such as... 

B, 90 min 95-100% buffer B, 95 min. Four to six abundant calcium-binding proteins, including recoverin, calmodulin, Capl and S-1006, are purified by... 

Retinal calcium-binding proteins obtained from the phenyl-Sepharose column can be separated by RP-HPLC (20). Calmodulin elutes first, followed by Capl. S-1006 elutes as two distinct peaks, while recoverin was separated into a series of at least three overlapping peaks (20). Mass spectrometry revealed that these peaks correspond to the different fatty acid modified forms of recoverin. The first peak contains the C14 2 and C12 0 acylated forms. The second recoverin peak consists of the C14 l species and the third peak C14 0. The same procedure may be used to isolate similarly modified proteins, including the photoreceptor G protein transducin. 

Figure 4. Calcium binding parameters for recoverin (27/iM) purified by phenyl-Sepharose and Mono Q chromatography (right panel).

Flu. 7. A schematic diagram of the conformational change and extrusion of the myri-stoyl group from the N-terminal domain of recoverin upon calcium binding followed by insertion into the membrane. 

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