Reconstitution of Nitrite Oxidation System

However, no one has succeeded in the reconfirmation of the results obtained by Aleem et al. (1963) on the reduction of NAD(P)+ with ferrocytochrome c. Freitag and Bock (1990) have reported that as the reduction of NAD+ with nitrite in Nitrobacter is not inhibited by DCCD which inhibits F0F -ATPasc, i.e., inhibits energy transfer through cytoplasm membrane (in the case of bacteria), the reduction of NAD+ is not performed by using the energy of ATP. They claim that nitric oxide participates in the formation of NADH, and the ratio of nitric oxide consumed/ 

NADH formed is 2.3. However, the mechanism by which NAD+ is reduced by nitric oxide is not as yet known. 

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