Recombinant genomic libraries

According to the opponents, the main argument against granting a patent was the lack of novelty of the recombinant DNA sequences claimed, which included the DNA sequences that coded interferon-alpha (IFN-alpha) as an insert. This prior art referred to DNA genome libraries, which are fractioned segments (15-20 Kb) of chromosomal DNA of human fetuses, cloned as derivatives of recombinant X phages. 

DNA (section 2.4.1) than eukaryotic RNA (section 2.4.2). The result will be the construction of a collection of cloned DNA fragments propagated in bacteria that is called the genomic library. This library should contain representatives of every sequence in the chromosome of a prokaryotic cell and every expressed gene in the case of a eukaryotic cell. The final step consists of the screening of every recombinant clone to identify the required gene(s). 

Once the genomic library has been generated it is necessary to screen for the gene of interest within a population of thousands of recombinant clones. The choice of screening method will very much depend on the availability of reagents and the information on the target gene to be isolated. 

The fragments are then randomly incorporated into vectors and the recombinant molecules are introduced into cells such as E. coli. The collection of these cells is called a genome library. 

---