Recombinant DNA biotechnology

There are other uses for antibodies that make use of their unique configurations. Producing useful quantities of antibodies can become the next challenge to be overcome. Recombinant DNA biotechnology is often used with bacteria or other organisms to produce specific antibodies that can be separated and purified for later use. 

Biotechnology is being appHed in the dairy industry. A significant and controversial development is the technique of producing transgenic animals, ie, animals in which hereditary deoxyribonucleic acid (DNA) has been augmented by DNA from another source, using recombinant DNA (rDNA) techniques. 

Microbes for Industiial and Agiicultural Applications, Dekker, NY, 1993. Click, B. R. and J. J. Pasternak, Molecular Biotechnology Trinciples and Applications of Recombinant DNA, ASM Press, Herndon, VA, 1994. Bajpai, Rakesb K., and Ales Prokop, eds. Recombinant DNA Technology II, Annals of the New York Academy of Sciences, vol. 721, 1993. 

Glick B.R Pasternak J.J. (1994) Molecular Biotechnology Principles and Applications of Recombinant DNA. Washington, D.C. American Society for Microbiology. 

The above two processes employ isolated enzymes - penicillin G acylase and thermolysin, respectively - and the key to their success was an efficient production of the enzyme. In the past this was often an insurmountable obstacle to commercialization, but the advent of recombinant DNA technology has changed this situation dramatically. Using this workhorse of modern biotechnology most enzymes can be expressed in a suitable microbial host, which enables their efficient production. As with chemical catalysts another key to success often is the development of a suitable immobilization method, which allows for efficient recovery and recycling of the biocatalyst. 

Biotechnology era beginning First recombinant DNA products Human insulin Human growth hormone Interferons, etc. Monoclonal antibodies Nucleotide blockage Growth in use of natural products and neutraceuticals... 

Bristow, A. 1993. Recombinant DNA derived insulin analogues as potentially useful therapeutic agents. Trends in Biotechnology 11, 301-305. 

Kingdon, H. and Lundblad, R. 2002. An adventure in biotechnology the development of haemophilia A therapeutics - from whole blood transfusion to recombinant DNA technology to gene therapy. Biotechnology and Applied Biochemistry 35, 141-148. 

In the late 1970s, development of recombinant DNA products utilizing knowledge of cellular and molecular biology commenced. The biotechnology industry became a reality. 

Click BR, Pasternak JJ, In Molecular Biotechnology Principles And Applications of Recombinant DNA (Eds Click BR, Pasternak JJ), pp. 145-169. American Society for Microbiology, Washington DC, 1998. 

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