Recombinant 2-deoxyribose 5-phosphate aldolase

A procedure for large-scale production of 2-deoxy-5-thio-D-eryf/tro-pentose (O Scheme 15) has been developed. It uses a recombinant 2-deoxyribose-5-phosphate aldolase (DER A) from E. coli strain DH5a as the catalyst that combines acetaldehyde with racemic 3-thioglyceralde-hyde [98]. 

C.-H. Wong, E. Garcia-lunceda, L. Chen, O. Blanco, H.I.M. Gijsen, D.H. Steensma, Recombinant 2-deoxyribose-5-phosphate aldolase in organic synthesis use of sequential two-substrate and three-substrate aldol reactions, I. Am. Ghem. Soc. 117 (1995) 3333-3339. 

This chapter describes recent developments in carbohydrate synthesis using microbial aldolases. Recombinant type II fructose-1,6> diphosphate aldolase and type I 2-deoxyribose-5-phosphate aldolase have been exploited for the synthesis of monosaccharides and analogs. A new procedure for the synthesis of dihydroxy acetone phosphate has been developed. Sialic acid aldolase has been used in conjunction with other enzyme-catalyzed modifications of monosaccharides for the synthesis of sialic acid-related sugars. 

Figure 7. Plasmid construction for the preparation of recombinant deoxyribose-5-phosphate aldolase (DERA). phosphopentomutase and nucleoside phosphorylase.

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