Recognition and binding of proteins

Developing molecularly imprinted polymers as receptors for recognising and binding proteins has practical applications (see also Chapter 10). Such polymers can be used as attractive materials for down stream processing of important 

Arnold and co-workers attempted to prepare imprinted metal-coordinating polymers for proteins [25]. For this purpose, efforts were made to prepare metalcoordinating molecularly patterned surfaces in mixed monolayers spread at the air-water interface or liposomes. This approach was termed as molecular printing and is illustrated in Fig. 6.6. In this process, a protein template is introduced into the aqueous phase, which imposes a pattern of functional amphiphiles in the surfactant monolayer via strong interactions with metal-chelating surfactant head groups. The pattern is then fixed by polymerising the surfactant tails. The technique has also been employed for two dimensional crystallisation of proteins [26]. 

Mosbach et al. also attempted to use metal-coordination interaction to prepare polymeric adsorbents for selective recognition of proteins [27]. Using the monomer (3), they polymerised the protein-monomer complex on methacrylate-derived silica particles in the presence of ribonuclease A as the template protein. After removing the template, the imprinted functionalised silica particles were used as packing 

Create mixed monolayer with functional amphiphile free to difftiije in two dimensions 

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Arnold and co-workers proposed a novel and generic approach to prepare mole-cularly imprinted matrices for the selective recognition and binding of proteins [40], They used metal coordination interaction to induce complementary binding... 

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