Receptors cloning

Glucocorticoid. Adrenal steroid receptors have been subdivided into two categories, one of which is the classical glucocorticoid receptor [7,19]. This receptor, cloned from human and rat sources, consists of a steroid- and DNA-binding subunit of 95 kDa [7, 19]. Such receptors, which have dissociation constants of 4-5 nM for glucocorticoids, are widely distributed across brain regions and are found in neurons and glial cells [7,19]. 

An alternative approach to clone the receptor was taken by Thomas and colleagues (1990, 1991). They screened a cDNA library from peritoneal rabbit cells with an antisense oligonucleotide probe deduced from the coding region of the second transmembrane domain, which is common to G-protein-coupled receptors (fMet-Leu-Phe is a G-protein-linked receptor). Screening of this library yielded a putative receptor clone F3R, characterised as follows ... 

Hsieh, D.J. and Liao, C.F., Zebrafish M2 muscarinic acetylcholine receptor cloning, pharmacological characterization, expression patterns and roles in embryonic bradycardia, Br. J. Pharmacol, 137, 782-792, 2002. 

Lee YM, Beinbom M, McBride EW, et al The human brain cholecystokinin-B/gastrin receptor cloning and characterization. J Biol Chem 268 8164-8169, 1993 Legris P, George Y, Boval P, et al A comparative study of alpidem versus Buspirone, in Imidazopyridines in Anxiety Disorders A Novel Experimental and Therapeutic Approach. Edited by Barthohni G, Garreau M, MorseUi PL. New York, Raven, 1993, pp 183-192... 

Auchampach JA, Jin X, Wan TC, Caughey GH, Linden J (1997) Canine mast cell adenosine receptors cloning and expression of the A3 receptor and evidence that degranulation is mediated by the A2B receptor. Mol Pharmacol 52 846-860... 

Auchampach JA, Jin X, Wan TC, Caughey GH, Linden J (1997) Canine mast cell adenosine receptors cloning and expression of the A3 receptor and evidence that degranulation is mediated by the A2B receptor. Mol Pharmacol 52(5) 846-860 Aune TM, McGrath KM, Sarr T, Bombara P, Kelley KA (1993) Expression of 5HTla receptors on activated human T cells. Regulation of cyclic AMP levels and T cell proliferation by 5-HT. J Immunol 151 (3) 1175-1183... 

Angiotensin II type 1 receptor cloned, sequenced, and mapped to chromosome 3q21-25 30... 

Sulfonylurea receptor cloned, associated with hyperinsulin secretion maps to chromosome 1 lpl5.1 38... 

Maroteaux L, Saudou F, Amlaiky N, Boschert U, Plassat JL, Hen R. Mouse 5HT1B serotonin receptor cloning, functional expression, and localization in motor control centers. Proc Natl Acad Sci USA 1992 89 3020-3024. 

Johnson MP, Baez M, Kumar JD, Nelson DL. Species differences in 5-HT2A receptors cloned pig and rhesus monkey 5-HT2A receptors reveal conserved transmembrane homology to the human rather than rat sequence. Biochim Biophys Acta 1995 1236 201-206. 

Blondel O, Vandecasteele G, Gastineau M, et al. Molecular and functional characterization of a 5-HT4 receptor cloned from human atrium. FEBS Lett 1997 412 465-474. 

Claeysen S, Faye P, Sebben M, Taviaux S, Bockaert J, Dumuis A. 5-HT4 receptors cloning and expression of new splice variants. Ann NY Acad Sci 1998 861 49-56. 

Matthes H, Boschert U, Amlaiky N, et al. Mouse 5-hydroxytiyptamine5A and 5-hydroxytryptamine5B receptors define a new family of serotonin receptors cloning, functional expression, and chromosomal localization. Mol Pharmacol 1993 43 313-319. 

Stam NJ, et al. Human serotonin 5-HT7 receptor cloning and pharmacological characterisation of two receptor variants. FEBS Lett 1997 413(3) 489-494. 

A second strategy used cloning by homology. Although this approach undoubtedly identified opioid receptor clones, it did not reveal the identity of opioid receptors and claims were retrospective. The approach was based on the assumption that the opioid receptors would be G protein-coupled receptors, and as indicated above, earlier pharmacological evidence strongly... 

---