Reagent removal

The efficiency of reagent removal from the capillary gap was assessed by filling capillary gaps between blank slides with peroxidase-streptavidin complex. After picking up the solution, the slide pairs were blotted for 1 min. The slide pairs were then subjected to three changes in buffer, followed by three, 1-min... 

The methylthio group in (4) can be substituted by Grignard reagents, removed by Raney nickel, or hydrolysed into a carbonyl group to yield a variety of furans and butenolides in fair to good yields. 

Grignard reagents remove tellurium from the tellurophene ring in refluxing benzene in the presence of nickel-phosphine complexes7. 

Interestingly, the same reagent removes S from abcPS with retention it is presumed that the first step is similar, but that SiCl3 then attacks sulfur, rather than phosphorus ... 

Although many oxidizing reagents remove the chromium tricarbonyl group, benzylic alcohols can be oxidized to benzaldehydes using dimethyl sulfoxide with acetic anhydride, trifluoroacetic anhydride, or sulfurtrioxide with minimal decomplexation. Asymmetric oxidation of aUcylthio-substituted complexes can be achieved using titanium tetraisopropoxide and an optically active tartrate ester (Scheme 108). Dimethyloxirane can also be used to oxidize sulfides to sulfoxides. 

Cleaning Up Combine all reaction mixtures in a beaker, add a few drops of acetone to destroy any unreacted iodine in potassium iodide reagent, remove the iodoform by suction filtration, and place it in the halogenated organic waste container. The filtrate can be flushed down the drain after neutralization (if necessary). 

MEK, 2-butanone, methyl ethyl ketone MEMO, 2-methoxyethoxymethylchloride Mercurochrome, mercury dibromofluorescein MES, 2-(7V-morpholino)-ethanesulfonic acid MESNA, Na 2-mercaptoethanesulfonate Metal impurities, by complexation by ion exchange resins by precipitation removal by extraction removal from reagents removal of lead removal of iron Methylarenes... 

PrrC (phenylisothiocyanate) UV 254 nm 1 pmol 15-27 min precolumn Salt interference, requires refrigeration, excess reagent removed under vacuum Ease of use, flexibility, good separation, reprodudbU-ity enhanced with automation... 

Aldehydes. Conversion to solid sodium bisulfite addition product with excess reagent, removal of nonaldehydic material by washing with alcohol or ether, and regeneration usually with acid, base, or sodium carbonate, provides a convenient method of purification. Examples syringic aldehyde, n-hexaldehyde. ... 

Reagents Consume water with excess reagents Remove water by azeotroping... 

Preparation of Extracts. Leffler (L2) and others have previously used isopropanol as a lipid solvent for determining cholesterol. Connerty et al. (C6) and Lofland (L4) also showed that triglycerides and phospholipids are extracted quantitatively. Under our extraction conditions, complete recovery of these lipid fractions is obtained at a serum-to-solvent ratio of 1 10 through 1 25. We routinely use 1 20 dilution (0.5 ml of sera+ 9.5 ml of isopropanol). We find that the addition of Lloyd s reagent removes bilirubin and other chromogenic material present in serum, as well as approximately 80% of the phospholipids present. 

Prevention of gum in certain organic liquids Storage of toxic volatile laboratory reagents Removal of certain catalyst residues... 

Acetylene precursors. Flash vacuum pyrolysis of these stabilized Wittig reagents removes triphenylphosphine oxide to furnish acetylenes. Both terminal and internal acetylenes are accessible by this method. 

Monodebromination of gem-dibromocyclopropanes. In conjunction with diethyl phosphonate or triethyl phosphite, the reagent removes one bromine atom in a highly stereoselective manner. 

Functional group Amino acid Protected derivative Reagent Removal... 

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