Rapid sequence screening

RS Youngquist, GR Fuentes, MP Lacey, T Keough. Generation and screening of combinatorial peptide libraries designed for rapid sequencing by mass spectrometry. J Am Chem Soc 117 3900-3906, 1995. 

Youngquist, R.S. Fuentes, G.R. Lacey, M.P Keough, T. Generation and Screening of Combinatorial Peptide Libraries Designed for Rapid Sequencing by Mass Spectrometry, J. Am. Chem. Soc. 117, 3900-3906 (1995). 

To obtain the sequence for CBP, a cDNA library from the silk gland was prepared and screened using a anti-CBP antibody. One positive clone was identified from 200,000 plaques, and rapid amplification of 5 complementary DNA ends (5 -RACE) was performed to obtain the full length of the CBP cDNA (Figure 24.2a). The predicted sequence encodes a 297-residue polypeptide of... 

ExPASy (Expert Protein Analysis System, www.expasy.ch) or the National Centre for Biotechnology Information (NCBI, www.ncbi.nlm.gov) websites. Both websites provide bioinformatics tools, links to sequence databases and extensive bibliographic resources. As an example of the wealth of information available on individual enzymes, at the time of writing a search based on nitrilase in the Entrez protein section of NCBI will recover more than 10000 references to nitrilase enzyme amino acid sequences. These can be rapidly screened online by organism, and the individual entries will have links to amino acid and gene sequence, relevant literature and information on protein features (such as conserved domains). 

---