Rainbow Trout Egg Polysialoglycoproteins

Developmental Expression of an a2,8-Polysialyltransferase Activity during Oogenesis 

The first ST, an a-N-acetylgalactosaminide (R- Gaipi 3GalNAcal O-Ser/Thr) a2,6-sialyltransferase (a2,6-ST), catalyzed incorporation of the first 

The a2,8-polysialosyl sialyltransferase (a2,8-polyST) catalyzed the third reaction, 

The a2,6- and a2,8-sialyltransferase activities appeared to be associated with distinct polypeptide chains because they could be differentiated by their stability toward freezing and thawing. Greater than 90% of the a2,6-ST activity, for example, remained after freezing and thawing. In contrast, the a2,8-ST and a2,8-polyST activities were lost after freezing and thawing. 

Using the CTP CMP-KDN Cytidylyltransferase from Rainbow Trout Testis 

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