Radiofrequency Thermal Damage of Cells

Induced heat can also be used control protein folding. For example, Kogan et al. modified gold nanospheres with a peptide, H-Cys-Leu-Pro-Phe-Phe-Asp-NH2, that binds to the amyloid (3 (A(3) protein and interferes with the growth of AP aggregates. The peptide-modified gold nanospheres bound to free APi 2 protein. 

Figu re 8.2 Structure of PEG- and amine-modified gold nanoparticles. The gold nanoparticles were prepared by NaBH4 reduction of HAuCE in the presence of 2-aminoethanethiol and mPEG-SH. 

Left lobe Median lobe Right lobe Caudate lobe 

Naked DNA (b) and DNA complexes with the nanoparticles (c) were injected into the mice, and electric pulses applied to the left lobe of the liver at 5 min after injection. Gene expression was evaluated at 24 h after injection the stained areas (black) in panel (c) correspond to the gene-expressing cells. RED = relative light unit. 

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