Radioactive zones autoradiography detection

A phosphor screen can be used like an autoradiography film to detect radioactive zones on PLC plates. The available screens are sensitive to x-rays and beta and gamma emissions from isotopes such as H, bij 32p 33p -pjjg screen captures... 

Radioactive zones can be detected on thin layers by film autoradiography, digital autoradiography with a multiwire proportional chamber, use of charged-coupled devices, or bioimaging/phosphor imaging techniques. These methods differ in terms of factors such as simplicity, speed, sensitivity, resolution, linear range, and accuracy and precision of quantification, and the method of choice depends on the available instrumentation, the type of experiment, and the information needed. 

TLRC can be used for animal, human, and plant metabolism analysis radiochemical purity and stability assessment toxicology and biochemical studies and separation, detection, and quantification of separated radioactive zones of all compound classes. Traditional film autoradiography and LSC continue to be widely used, but phosphor imaging and layer scanners are being increasingly applied. The instruments for these methods are highly automated and... 

When properly exposed, the resolution of the zones in autoradiography can be comparable to that on the original chromatogram and as high as any other radioactivity detection method. The major disadvantage is that exposure times can vary from a few hours to several weeks, depending on the amount of radioactivity in the TLC zones. Various commercial films are used, one of the newest and most sensitive being Biomax MR from Kodak (Rochester, New York, U.S.A.). 

Environmental radioactivity resulting from atmospheric nuclear-weapons testing is detectable in all components of the ecosystem. Autoradiography as a method of detection was utilized by Hawthorn and Duckworth (1958) in studying the presence of radioactive strontium in deer antlers. A 2-mm transverse section of the antler was placed on X-ray film and exposed for 82 days. The autoradiograph disclosed a concentration of activity in the peripheral zone of the antler (Figure 4). 

---