Quantum dot conjugation

Babu, P., Sinha, S., and Surolia, A. (2007) Sugar-quantum dot conjugates for a selective and sensitive detection of lectins. Bioconjugate Chem. 18, 146-151. 

Tada, H., Higuchi, H., Wanatabe, T.M., and Ohuchi, N. (2007) In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice. Cancer Res. 67, 1138-1144. 

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Label the samples by immersion into 5 nM secondary quantum dot conjugate, such as goat anti-mouse 655 nm quanmm dots, in blocking buffer. Incubate for 30 min at room temperature. 

Fig. 3. Immune electron micrographs of quantum dot-labeled bacteria. Mixtures of cultured HeLa cells and Chlamydia trachomatis elementary bodies (EB) were prefixed, probed with anti-EB rabbit serum, and labeled with goat anti-rabbit IgG/655 nm quantum dot conjugates. Thin sections examined by TEM (A) and cell layers examined by SEM (B) showed numerous quantum dots on bacterial surfaces, but not on host cells (unpublished data). Bars, 50 nm.

Chen, H., Titushkin, L, Stroscio, M., Cho, M. Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells. Biophys. J. 92, 1399-1408 (2007)... 

Bhang SH, Won N, Lee T et al (2009) Hyaluronic acid-quantum dot conjugates for in vivo lymphatic vessel imaging. ACS Nano 3 1389-1398... 

So MK, Xu C, Loening AM et al (2006) Self-illuminating quantum dot conjugates for in vivo imaging. Nat Biotechnol 24 339-343... 

Ba , D. and Boyaci, l.H. (2011) Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides. Anal. Bioanal. Chem., 400 (3), 703-707. 

Copper-free dick chemistiy for highly luminescent quantum dot conjugates application to in vivo metabolic imaging." Bioconjugate Chemistry, Vol.21, No.4, (Apr 21,2010), pp. 583-588, ISSN 1520-4812... 

The photodynamic activity of QD and QD-PS conjugates was first explored by Sarnia et al. who investigated a phthalocyanine - CdSe quantum dot conjugate. Their results showed that QDs are capable to directly photosensitise the production of 02, albeit with a low quantum yield, e.g.,

toluene solution. However, when conjugated to a Si phthalocyanine efficient FRET to the phthalocyanine takes place, followed by energy-transfer from the phthalocyanine to 2. The low ability of QDs to directly produce 2 was confirmed in a number of additional studies (Table... 

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