N-Acetylglucosamine transferase

Whelan SA, Hart GW. Proteomic approaches to analyze the dynamic relationships between nucleocytoplasmic protein glycosylation and phosphorylation. Circ. Res. 2003 93 1047-1058. Konrad RJ, Zhang F, Hale JE, Knierman MD, Becker GW, Kudlow JE. Alloxan is an inhibitor of the enzyme O-linked N-acetylglucosamine transferase. Biochem. Biophys. Res. Commun. 2002 293 207-212. 

Algl3, the sugar donor subunit of a yeast N-acetylglucosamine transferase 202 117 362... 

Using the activated GlcNAc-UDP as substrate, the enzyme N-acetylglucosamine-transferase I then attaches an N-acetylated glucosamine ... 

Yki-Jarvinen, H. Vogt, C. Lozzo, P. Pipek, R. Daniels, M.C. Virkamaki, A. Makimattila, S. Mandarine, L. DeFronzo, R.A. McClain, D. Gottschalk, W.K. UDP-N-acetylglucosamine transferase and glutamine Fructose 6-phosphate amidotransferase activities in insulin-sensitive tissues. Diabetologia 1997, 40, 76-81. 

N-Acetylglucosamine transferase Other specific glycosyl transferases Thiamine pyrophosphatase... 

Raetz, C. R., and Roderick, S. L. (1995). A left-handed parallel beta helix in the structure of UDP-N-acetylglucosamine acyl transferase. Science 270, 997-1000. 

Sulzenbacher, G., Gal, L., Peneff, C., Fassy, F., and Bourne, Y. (2001). Crystal structure of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyl transferase bound to acetyl-coenzyme A reveals a novel active site architecture. J. Biol. Chem. 276, 11844-11851. 

No data are available regarding effects of competing enzymes on the activities of transferases other than glucuronyltransferase. For assays of native transglucuronylation rates, it may be useful to run parallel incubations with UDP-N-acetylglucosamine-fortified (SIO) and unfortified preparations, particularly when species are compared. 

T Skarzynski, A Mistry, A Wonacott, SE Hutchinson, VA Kelly, K Duncan. Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin. Structure 4 1465-1474, 1996. 

M Crouvoisier, D Mengin-Lecreulx, J van Heijenoort. UDP-N-acetylglucosamine N-acetylmuramoyl-(pentapeptide) pyrophosphoryl undecaprenol N-acetylglucos-amine transferase from Escherichia coli. overproduction, solubilization, and purification. FEBS Lett 449 289-292, 1999. 

With the onset of lactation, a-lactalbumin is formed in the mammary gland and alters the substrate specificity of the transferase from N-acetylglucosamine to glucose, enabling lactose synthesis to be effected ... 

To date commercial /3(l-4)ga/actosyl transferase from bovine milk - /J(l-4)GalT -is the most thoroughly studied transferase with respect to its scope of the acceptor- and donor-specificities [28, 48]. The enzyme transfers a D-galactose unit from UDP-Gal onto the 4-OH-group of a terminal N-acetylglucosamine acceptor in a /1-mode to form N-acetyllactosamine (Fig. 3). In the presence of a-lactalbumine, the preferred acceptor is glucose to give lactose, respectively. 

Formation of starting materials for cell wall synthesis begins with two metabolic substances normally found in all life forms N-acetylglucosamine 1-phosphate and the pyrimidine nucleotide uridine triphosphate (UTP) (see Fig. 6-3). Condensation of these two compounds by elimination of pyrophosphate affords uridine-diphospho-N-acetylglucosamine (UDPNAG). Reaction with phosphoenolpyruvic acid (PEP, the activated form of the enol tautomer of pyruvic acid),5 catalyzed by a specific transferase, yields the 3-O-enolic ether. 

Kluyvevomyces tactics that secretes mannan lacking the terminal al 2-linked N-acetylglucosamine units even though cell extracts show the wild-type transferase activity when assayed with an exogenous acceptor (3 ) Alternative explanations for this observation have been considered (Table II), and it still may develop... 

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