Purine metabolism, effect fructose

The present investigation was undertaken in an attempt to further clarify the effects of fructose on purine nucleotide metabolism. Isolated rat hepatocytes were chosen as a model system, since they allow the study of each pathway of purine metabolism separately and without interference from other tissues. 

EFFECTS OF FRUCTOSE ON PURINE NUCLEOTIDE METABOLISM IN ISOLATED RAT HEPATOCYTES... 

In recent years it became apparent that fructose metabolism in the liver tissue has considerable effects on purine nucleotide metabolism. There is ample evidence that fructose metabolism is associated with acceleration of nucleotide degradation (1-5) and evidence is accumulating (6-8), indicating an acceleration effect of fructose also on purine synthesis. 

Fig, 1. - Effect of fructose on isolated rat hepatocyte.s content of ATP, PP-rib-P and Pi, on the rate of de novo purine synthesis (( C) formate incorporation) and on PP-rib-P availability for metabolic reaction (( C) adenine incorporation). The time of incubation indicates the time of incubation with 28mM glucose or fructose before the assay of the specified parameter. The measurement of the rate of incorporation of ( C) formate and of ( C) adenine required additional incubation of 30 min, following the incubation period indicated in the figure. Thus, for these two measurements, 0 time of incubation values refer to values obtained during the 30 min assay incubation period alone (without preincubation). 0 - incubation with glucose o - incubation with fructose. 

---