Purification of callose and cellulose synthases

When a reliable assay method is available for glucan synthases, and once it has been demonstrated that the in vitro products were synthesized de novo and that they correspond to the expected polysaccharides, biochemical approaches can be used to obtain enriched enzyme preparations. The reports 

The development of highly sensitive proteomic methods for the characterization of membrane-bound proteins should facilitate the systematic sequencing of all the proteins that are present in fractions with high callose synthase activity. Also, the progress made on in vitro synthesis of cellulose is promising and proteomic analysis may also be used on fractions enriched in cellulose synthases in the near future. This approach combined with detailed structural characterization of in vitro products, immunochemical methods, molecular biology and gene 

Arioli T., Peng L., Betzner A.S., Bum X, Wittke W., Herth W., Camilleri C., Hofte H., Plazinski X, Birch R., Cork A., Glover X, Redmond X., and Williamson R.E. 1998. Molecular analysis of cellulose biosynthesis in Arabidopsis. Science 279 717 720. 

Atalla R.H. and VanderHart D.L. 1984. Native cellulose a composite of two distinct crystalline 

The biosynthesis of cellulose. J Macromol Sci Pure Appl Chem 10 1345-1373. 

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