Psilocybin growth

There has been much mention of this test in the psychedelic literature, but it is by no means reliable. Some mushrooms blue only after the first "flush of the fruiting bodies (growth of the fruiting bodies recurs, usually at one-week intervals). Some authors have concluded that this "bluing occurs only in mushrooms containing both psilocin and psilocybin. 

There were no "bad trips" in those days. We didn t know what a "bad trip was. Hundreds of psilocybin trips—I never saw one. I didn t even have a word "bad trip in my vocabulary. Those were benign, life-changing, growth... 

The process of growth can be followed with a saccharimeter. The maximum growth and highest percentage of psilocybin occurs about four days after all of the broth s sugar content has been used up. The mycelium should be harvested at this time. Any jars that cannot be harvested on that day should be refrigerated until this can be done. 

Three brassinosteroids have been used to speed up the life cycle of fungi in vitro. These are brassinolide, 24-epibrassinolide, and 22S,23S-homobrassinolide all of which increased mycelial growth by a factor of 2-3 and induced earlier sporocarp formation in Psilocybe cubensis and Gymnopilus purpuratus (20). P. cubensis forms part of the group known as the sacred mushrooms employed satisfactorily in religious ceremonies by Mexican Indians. One of the biologic y active compounds contained in Psilocybe is psilocybin which has been isolated, synthesized and used in the study of schizophrenia (19). The effects with 22S,23S-... 

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