Pseudomonas sp. NCIB

Organism bovine heart spinach Pseudomonas sp. NCIB 9816-4 E. coli (expression)... 

The use of resting cells of microorganisms to biotransform organic compounds by specific reactions is a well-established technique. Thus, incubation of norbornanone (12) with a washcd-cell suspension of cyclopentanol grown Pseudomonas sp. NCIB 9872 resulted in the rapid oxidation of the substrate to a mixture of lactones 13 and 14398. 

Biodegradation of 2,4-D is affected strongly by the pH value of the culture. At a pH of <5.1, there is extensive accumulation of its metabolite, 2,4-dichlorophenol, which inhibits the biodegradation completely (Sinton et al. 1987). The authors noted that biodegradation ceased completely when the concentration of the metabolite reached 44 mg/L. The highest growth rates (Pseudomonas sp. NCIB 9340) occurred at pH... 

The culture Pseudomonas sp. NCIB 9872 was isolated from soil using cyclopentanol as the sole carbon source [9]. Cell-free extracts of the organism displayed alcohol dehydrogenase activity and BVase activity. To obtain energy from this carbon source, the pivotal step is ling fragmentation, made possible by oxidation of the alcohol to the cyclopentanone, followed by insertion of oxygen atom to S-valerolactone, and subsequent enzymatic hydrolysis to aliphatic hydroxy acids which are then converted to metabolites. The purified enzyme consists of four subunits (M 200,000), binds two to four molecules of FAD, and requires NADH as reductant. 

The first exploitation of microbial BVase to produce chiral lactones from cyclic racemic bicyclo[2.2.1]hept-2-en-7-ones (Fig. 10) was reported by Ouazzani et al. [17]. A report of preparative scale bioconversion of cyclic ketone was made [26]. The preparative scale bioconversion of using Acinetobacter TD 63 provides lactones in high optical purity [27]. Subsequently [28], whole-cell bioconversions of bicyclic lactones was made, using Acinetobacter sp. NCIB 9871 and Pseudomonas sp. NCIB 9872 to produce file same chiral synthons. In all cases, the racemic ketone was oxidized, and equal amounts of the regioisomeric lactones (—)-(i5, 5/ )-2-oxabicyclo[3.3.0]oct-6-en-3-one and (—)-(iR,55)-3-oxabicyclo[3.3.0]oct-6-en-3-one were produced in high enantiomeric purity. 

Lee K, DT Gibson DT (1996) Toluene and ethylbenzene oxidation by purified naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4. Environ Microbiol 62 3101-3106. 

Larkin Ml (1988) The specificity of 1-naphthol oxygenases from three bacterial isolates, Pseudomonas spp. (NCIB 12042 and 12043) and Rhodococcus sp. (NCIB 12038) isolated from garden soil. EEMS Microbiol Lett 52 173-176. 

Resnick SM, DT Gibson (1996) Regio- and stereospecific oxidation of fluorene, dibenzofuran, and dibenzothiophene by naphthalene dioxygenase from Pseudomonas sp. strain NCIB-4. Appl Environ Microbiol 62 4073-4080. 

The transformation of carbazole has been examined, and naphthalene 1,2-dioxygenase activity was induced in Pseudomonas sp. strain NCIB 9816-4 and in Beijerinckia sp. strain B8/36 (Resnick et al. 1993). The 3-hydroxycarbazole that was formed could have resulted from two pathways (a) from the initial production of ciy-3,4-dihydro-3,4-dihydroxycarbazole followed by dehydration or (b) by monooxygenation that cannot be excluded since monooxygenase activity can be mediated by naphthalene 1,2-dioxygenase (Gibson et al. 1995). 

Ensley, B. D., Gibson, D. T. Laborde, A. L. (1982). Oxidation of naphthalene by a multicomponent enzyme system from Pseudomonas sp. strain NCIB 9816. Journal of Bacteriology, 149, 948-54. 

Naphthalene 1,2-dioxygenase activity induced in Pseudomonas sp. strain NCIB 9816-4 and from Beijerinckia sp. strain B8/36 by incubation with carbazole produced 3-hydroxycarbazole (Resnick et al. 1993). Although this product could have resulted from the initial... 

The enzymes from Arthrobacter, Bacillus stearothermophilus NCIB 8224 and NS 1122A, and Pseudomonas sp. NS 671 have been cloned and expressed in E. coli. The enzymes from Bacillus and Pseudomonas share approximately 38% sequence identity with the Arthrobacter enzyme whereas the 20 amino acids known from the N-termini of the enzymes from Alcaligenes and Pseudomonas putida IFO 12996 are... 

Microbial degradation of nitrile compounds was performed by different microorganisms, capable of growing on various aliphatic and aromatic nitriles [107-109], They are degraded through two pathways (Figure 7) one is the direct hydrolysis of nitriles to carboxylic acid and ammonia, catalyzed by nitrilases. Nitrilases, that utilize benzonitrile and related aromatic nitriles as substrates, have been purified from Pseudomonas sp. [110.111], Nocardia sp. strains NCIB 11215 [112] and NCIB 11216... 

Seo J, Kang S-I, Ryu J-Y, Lee Y-J, Park KD, Kim M, Won D, Park H-Y, Ahn J-H, Chong Y, Kanaly RA, Han J, Hur H-G (2010) Location of flavone B-ring controls regioselectivity and stereoselectivity of naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4. Appl Microbiol Biotechnol 86 1451-1462... 

Purified CHMOs from Acinetobacter sp. NCIB 9871 and Pseudomonas NCIMB 10007 coupled with dehydrogenases to generate NADPH/NADH in situ was used to prepare (1R,5S)- and (i5, 5/ )-lactones [29,30]. These chiral lactones are important synthons used in the synthesis of prostaglandins. 

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