Pseudomonas putida NCIB

Stuart-Keil KG, AM Hohnstock, KP Drees, JB Herrick, EL Madsen (1998) Plasmids responsible for horizontal transfer of naphthalene catabolism genes between bacteria at a coal tar-contaminated site are homologous to pDTGl from Pseudomonas putida NCIB 9816-4. Appl Environ Microbiol 64 3633-3640. 

Feng Y, HE Khoo, CL Poh (1999) Purification and characterization of gentisate 1,2-dioxygenases from Pseudomonas alcaligenes NCIB 9867 and Pseudomonas putida NCIB 9869. Appl Environ Microbiol 65 946-950. 

Little is known about the hydroxylation of heterocyclic substrates, and more experimental work is needed in this area. Achromobacter xylosoxydans (DSM 2783) and Pseudomonas putida (NCIB 8176 or 10521) are particularly effective in the hydroxylation of nicotinic acid (107) to 6-hydroxynicotinic acid (108 equation 39), and commercial processes capable of giving up to 97% yield have been developed. It is interesting to note that in this case the product is precipitated as it forms by formation of its magnesium or barium salt, and this could serve to protect the product from further degradation as well as facilitating its recovery. 

Simon Ml, TD Osslund, R Saunders, BD Ensley, S Suggs, A Harcourt, W-C Suen, DL Cruden, DT Gibson, GJ Zylstra (1993) Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4. Gene 127 31-37. 

The enzymes from Arthrobacter, Bacillus stearothermophilus NCIB 8224 and NS 1122A, and Pseudomonas sp. NS 671 have been cloned and expressed in E. coli. The enzymes from Bacillus and Pseudomonas share approximately 38% sequence identity with the Arthrobacter enzyme whereas the 20 amino acids known from the N-termini of the enzymes from Alcaligenes and Pseudomonas putida IFO 12996 are... 

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