Pseudomonas flourescens

Obligate aerobe Bacteria which require the presense of oxygen, such as Pseudomonas flourescens. A few strains of this species are capable of utilizing nitrate to allow anaerobic respiration. 

An alternative way to mne the polymer properties and insert desired functionalities is copolymerization with different monomers. Cyclic carbonates have been copolymerized with various other cyclic monomers, such as lactones or lactides. For example, TMC was copolymerized with 5-methyl-5-benzyloxycar-bonyl-l,3-dioxan-2-one (MBC) using lipase from Pseudomonas flourescens (PF) resulting in a highly amorphous random copolymers (Fig. 8) [74]. In another smdy, 5-benzyloxy-trimethylene carbonate (BTMC) was copolymerized with 5,5-dimethyl-trimethylene carbonate (DTC) using an immobilized hpase on silica particles [79]. In the copolymerization of TMC with a lactone, m-pentadecalactone (PDL), employing Novozyme 435, highly erystalline TMC-PDL eopolymers were obtained, and as opposed to chemical catalysts, enzyme eatalyst (Novozyme 435) polymerized PDL more rapidly than TMC [80]. 

An illustrative example is the resolution of the carbon-14-labeled hydroxyindane derivative 11, an intermediate in the synthesis of the serotonin uptake inhibitor MDL 21111 A. Under catalysis by lipase from Pseudomonas flourescens, vinyl acetate reacted with the alcohol 11 in tert-butyl methyl ether solvent. After flash-chromatography on silica gel the acylated (12, 43% yield 97% e.e.) and nonacylated (13. 47% yield, 98% e.e.) compounds were obtained in excellent optical pmities. ... 

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