Pseudomonas cepacia ATCC

The Production of Poly-3-hydroxybutyrate-co-3-hydroxyvalerate with Pseudomonas cepacia ATCC 17759 on Various Carbon Sources... 

Strain Pseudomonas cepacia ATCC 17749 was stored under liquid nitrogen for longer storing periods at the institutes strain collection. For culture maintenance the strain was grown on solid media for two to three days at 30 °C on LB medium and on mineral medium with various carbon sources (glucose, galactose and lactose) respectively. Then the plates were kept at 4 °C. Fresh plates were inoculated every two to three weeks. 

Figure I. Fermentation on glucose as carbon source and production of P(3HB-co-3HV) with sodium valerate as precursor and Pseudomonas cepacia ATCC 17759...

Figure 4. Fermentation on glucose and galactose as carbon source with Pseudomonas cepacia ATCC 17759, development of kinetic data...

The use of extracellular lipases of microbial origin to catalyze the stereoselective hydrolysis of esters of 3-acylthio-2-methylpropionic acid in an aqueous system has been demonstrated to produce optically active 3-acylthio-2-methyl-propionic acid [41-43], The synthesis of the chiral side chain of captopril by the lipase-catalyzed enantioselective hydrolysis of the thioester bond of racemic 3-acetylthio-2-methylpropionic acid (15) to yield 5 -(-)-(15) has been demonstrated [44], Among various lipases evaluated, lipase from Rhizopus oryzae ATCC 24563 (heat-dried cells), BMS lipase (extracellular lipase derived from the fermentation of Pseudomonas sp. SC 13856), and lipase PS-30 from Pseudomonas cepacia in an organic solvent system (l,l,2-trichloro-l,2,2-tri-fluoroethane or toluene) catalyzed the hydrolysis of thioester bond of undesired enantiomer of racemic (15) to yield desired S-(-) (15), R-(+)-3-mercapto-2-methylpropionic acid (16) and acetic acid (17) (Fig. 8A). The reaction yield of... 

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