Protoplast culture

J. Spiral and co-workers, "Protoplast Culture and Regeneration in Coffea Species," in Ref. 40. 

Plant tissue and protoplast culture applications to stress physiology and biochemistry... 

Lin, W., Odell, J.T. Schreiner, R.M. (1987). Soybean protoplast culture and direct gene uptake and expression by cultured soybean protoplasts. Plant Physiology, 84, 856 1. 

Rea G, Metoui O, Infantino A, Federico R, Angelini R (2002) Copper amine oxidase expression in defense responses to wounding and Ascochyta rabiei invasion. Plant Physiol 128 865-875 Roeder V, Collen J, Rousvoal S, Corre E, Leblanc C, Boyen C (2005) Identification of stress gene transcripts in Laminaria digitata (Phaeophyceae) protoplast cultures by Expressed Sequence Tag analysis. J Phycol 41 1227-1235... 

Protoplast cultures. Cellular tissues devoid of cell wall material in culture... 

Protoplast cultures involve the growth of protoplasts in solid or liquid medium. Protoplasts can be prepared by mechanically or enzymatically removing the cell wall. The isolated protoplasts can be used (1) to modify the genetic information of plant cells,... 

Protoplast culture aseptic isolation and culture of plant protoplasts from cultured cells or plant tissues (66). This type of culture is usually employed to... 

Okanishi, M., Suzuki, K., and Umezawa, H. (1974) Formation and reversion of streptomycete protoplasts cultural condition and morphological study./. Gen. Microbiol, 80, 389-400. 

Maruyama, E., Hosoi, Y., and Ishii, K. (2002) Somatic embryogenesis in Sawara cypress Chamaecyparis pisifera Sieb. et Zucc.) for stable and efficient plant regeneration, propagation and protoplast culture. J. For. Res., 7 23-34. 

Klimaszewska, K (1989). Recovery of somatic embryos and plantlets from protoplast cultures of Larix x eurolepsis. Plant Cell Reports, Vol. 8, pp. 440-444. 

Carefully transfer the protoplasts to 5-cm sterile petri dishes containing 5 ml of protoplast culture medium. Seal the dishes with Parafilm and incubate in the dark at 24°C for 40 to 50 hr. 

Rains DW. Plant tissue and protoplast culture applications to stress physiology and biochemistry. In Jones HG, Flowers TJ, Jones M, editors. Plant under stress. Cambridge Cambridge University Press, 1989 181-186. 

Eruca sativa, also known as Rocket or arugula, is usually used as a salad component, but there is interest in using it as a lubricant or biodiesel crop (Li et al., 2009). The seeds contain approximately 45% erucic acid and about 9% gadoleic acid (C20 l) (Lazzeri et al., 2004). Plantlet regeneration via somatic embryogenesis (Chen et al., 2011), protoplast culture (Sikdar et al., 1987), and... 

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