Proteomics target identification

Chemical proteomics consists of the classical drug-affinity chromatography and modern high-resolution MS analysis for protein identification [3, 11]. The procedure typically involves immobilization of the compound of interest to a solid support through a spacer arm, and the affinity matrix is then used to purify specific interacting proteins from cellular lysate. The complex proteomic mixture is then proteolytically digested, and the resulting peptides are sequenced 

The first step in chemical-genetics-based target finding is to perform cellular phenotypic screening to identify compounds that elicit a desired phenotype. An array of orthogonal secondary assays is then performed to remove nonspecific 

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Kopec, K. K., Bozyczko-Coyne, D., and Williams, M. (2005). Target identification and validation in drug discovery The role of proteomics. Biochem. Pharmacol. 69 1133-1139. 

In the future, proteomics will play a major role in drug discovery, accelerating the various steps involved - target identification, target validation, and drug discovery (efficacy, selectivity and mode of action). Operated on a routine basis, MD-LC may provide the desired data, and after interconnection with the biology, solutions could be foimd. 

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