Proteomes enzyme target

In this platform, antibodies that specifically recognize enzyme targets of ABPP are arrayed on glass slides and used as capture reagents. The application of probe-labeled proteomes on these slides results in the localization of fluorescence, which can be directly detected by fluorescence scanning [50]. The end result for this experiment is the consolidation of the isolation, detection, and identification of probe-labeled enzymes into one step (Fig. 6). 

Activity-based protein profiling (ABPP) is a chemical proteomic strategy in which active-site-directed covalent probes are used to profile the functional states of enzymes in complex proteomes. Activity-based probes (ABPs) can distinguish active enzymes from their inactive zymogens or inhibitor-bound forms. They contain a reactive group intended to modify enzyme active sites covalently and a reporter group (typically rhodamine or biotin) that assists in detection and identification of protein targets. 

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