Proteins genome-wide analysis

McCraith, S., Holtzman, T., Moss, B., and Fields, S. (2000). Genome-wide analysis of vaccinia virus protein-protein interactions. Proc. Natl. Acad. Sci USA 97, 4879-4884. 

McCraith S et al. Genome-wide analysis of vaccinia virus protein-protein interactions. Proc Natl Acad Sci USA 2000 97 4879-4884. 

Wallin, E., and von Heijne, G. (1998). Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci. 7, 1029-1038. 

Jesch, S.A., Zhao, X., Wells, M.T., and Henry, S.A., 2005, Genome-wide analysis reveals inositol, not choline, as the major effector of Ino2p-Ino4p and unfolded protein response target gene expression in yeast. J. Biol. Chem. 280 9106-9118. 

Stephen McCraith, Ted Holtzman, Bernard Moss and Stanley Fields, Genome-wide analysis of vaccinia viius protein-protein interactions. Proceedings of the National Academy of Sciences USA, 97 (2000), 4879-4884. 

The putative kinase domains are not highly conserved over evolution. The N-terminal kinase domain has weak similarity to a protein kinase family, whereas the C-terminal domains shows no similarity to any known kinase. Further characterization of the N-terminal kinase domain indicates that acidic residues in two small regions are important for the kinase activity (O Brien and Tjian, 1998). Combined mutation of both regions disrupts kinase activity, and the mutant has reduced ability to rescue the ts 13 cell line, a ts hamster cell line with a mutation in TAFl. This mutant shows a defect in transcription of approximately 6% in a genome-wide analysis (O Brien and Tjian, 2000), indicating that the kinase activity of the N-terminal domain may be required for expression of a subset of genes in vivo. 

Analysis of genome-wide protein-protein interactions in yeast... 

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