Protein Analysis Methods

The consensus among proteome researchers is that separation is an essential part of complex protein analysis methods. A simplification of complex samples using 2DLC is beneficial for state-of-the-art MS/MS analysis. While 2DLC potentially provides a higher peak capacity than 1DLC, the orthogonality of separation has to be taken into consideration. 

D. Capillary Zone Electrophoresis for Protein Analysis METHOD TRANSFER... 

Los GV, Wood K. (2007) The HaloTag a novel technology for cell imaging and protein analysis Methods Mol Biol 356, 195-208. 

Christodoulides N et al (2005) Toward the development of a lab-on-a-chip dual-function leukocyte and C-reactive protein analysis method for the assessment of inflammation and cardiac risk. Clin Chem 51(12) 2391-2395... 

The essential slow modes of a protein during a simulation accounting for most of its conformational variability can often be described by only a few principal components. Comparison of PGA with NMA for a 200 ps simulation of bovine pancreatic trypsic inhibitor showed that the variation in the first principal components was twice as high as expected from normal mode analy-si.s ([Hayward et al. 1994]). The so-called essential dynamics analysis method ([Amadei et al. 1993]) is a related method and will not be discussed here. 

R. A. Copeland, Methods for Protein Analysis A Practical Guide to Eaboratoy Protocols, Chapman Had, New York, 1994. 

Ironically, the normal mode analysis method can be used to detennine properties of the multiple-minima energy surface of proteins. 

The second example is the SE-HPLC analysis of recombinant hGH. In this example, SE-HPLC is used for both a purity and a protein concentration method for bulk and formulated finished products. This method selectively separates both low molecular weight excipient materials and high molecular weight dimer and aggregate forms of hGH from monomeric hGH, as shown... 

Jiang X, Jiang X, Feng S, et al. Development of efficient protein extraction methods for shotgun proteome analysis of formalin-fixed tissues. J. Proteome Res. 2007 6 1038-1047. 

Phizicky, E.M., and Fields, S. (1995) Protein-protein interactions Methods for detection and analysis. Microbiol. Rev. 59, 94-123. 

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