Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Proteins activity screening, genomic librarie

The genome of the Helicobacterpylori bacterium is 1.6 million base pairs in size and encodes 1590 ORFs (Tomb et al., 1997). The comprehensive two-hybrid library screen performed with these ORFs differs from the yeast experiments described above in that the Gal4 activation domain library used consisted of over ten million random genomic fragments (Rain et al., 2001). Thus, the potential problem of full-size ORFs masking protein-protein interactions is reduced. A total of 261 ORFs were fused to the Gal4 DNA binding domain to create a set of baits. These ORFs... [Pg.58]


See other pages where Proteins activity screening, genomic librarie is mentioned: [Pg.83]    [Pg.298]    [Pg.177]    [Pg.100]    [Pg.39]    [Pg.425]    [Pg.72]    [Pg.161]    [Pg.1115]    [Pg.487]    [Pg.606]    [Pg.298]    [Pg.1]    [Pg.59]    [Pg.59]    [Pg.251]    [Pg.330]    [Pg.403]    [Pg.80]    [Pg.77]    [Pg.252]    [Pg.7]    [Pg.217]    [Pg.490]    [Pg.336]    [Pg.27]    [Pg.10]    [Pg.153]    [Pg.536]    [Pg.142]    [Pg.569]    [Pg.1726]    [Pg.53]    [Pg.164]    [Pg.2]    [Pg.106]    [Pg.460]    [Pg.638]    [Pg.85]    [Pg.220]    [Pg.828]    [Pg.933]    [Pg.954]    [Pg.20]    [Pg.21]    [Pg.146]    [Pg.102]    [Pg.104]    [Pg.20]    [Pg.21]   
See also in sourсe #XX -- [ Pg.425 ]




SEARCH



Genomic library

Genomic screening

Library screening

Library screening libraries

Screen libraries

© 2024 chempedia.info