Methods protein purification

E. L. V. Harris and S. Angal, eds.. Protein Purification Methods A Practical Approach, IRL Press at Oxford University Press, Oxford, U.K., 1989. 

Harris, E.L.V., Protein purification methods A Practical Approach . IRL Press, Oxford, 1989. 

Deutscher MP (editor) Guide to Protein Purification. Methods En-zymol 1990 182. (Entire volume.)... 

Rigaut G, Shevchenko A, Rutz B, et al. A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 1999 17 1030-1032. 

Deutscher MP. Guide to Protein Purification Methods in Enzymology, Academic Press, New York, 1990. 

These efforts are characterized by a specific protein purification method. The aim of the investigation is to find the molecules involved... 

Leave to express for 3-4 days, then collect medium, spin, filter 0.22 pm, then go on to protein purification (method depends on the tags used etc.). 

Harris EL, Angal S (1990) Protein purification methods a practical approach. Oxford Univ. Press, Oxford... 

M. Q. Xu, H. Paulus, and S. Chong, Fusions to self-splidng inteins for protein purification, Methods Enzymol. 2000, 326, 376-418. 

First, a general method of transformation of the expression plasmid into E. coli will be described, as well as appropriate conditions for growth and induction of the expression cultures. Then, the 96-well protein purification method is detailed. Last, analysis of the purified proteins is described using both lab-on-a-chip technology and traditional sodium dodecyl sulfide (SDS)-polyacryla-mide gel electrophoresis (PAGE). 

After cell cultivation, a cell separation step is generally needed to process the culture medium to purify the product. This step is performed either to obtain a cell-free medium for an extracellular product or to obtain a cell concentrate if the product is intracellular. In animal cell cultures, the product is normally secreted. Thus, after cell separation, the product is in solution and can be processed by chromatographic techniques or other protein purification methods, as presented in Chapter 12. 

A generic protein purification method for protein complex characterization and proteome exploration. 

Considerable purification of lipoxygenase from soybeans was achieved by Balls et al (15), They used conventional protein purification methods and the carotene oxidase reaction to follow the enzyme activity. Theorell et al, (16), in 1947, crystallized the enzyme, or more correctly, one of the several isoenzymes in soybean. We believe this was lipoxygenase-1 as described later. 

Deutseher, M.P., Ed. (1990) Protein Purification, Methods Enzym., Vol. 182, 894pp, Academic Press, San Diego, CA. 

For further details showing variation of % saturation versus temperature and a review of precipitation tachniques see Guide to Protein Purification, Methods in Enzymology, Vol. 182, p. 291 Acad. Press 1990. 

Hames, B. D. A Practical Approach. Oxford University Press. A methods series that describes protein purification methods and assays. 

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