Protein polymers, genetically engineered

Keywords Elastinlike polymers Genetic engineering Protein-based polymers -Self-assembly Smart polymers... 

Anderson, J. P., Cappello.J., and Martin, D. C. (1994). Morphology and primary crystal-structure of a silk-like protein polymer synthesized by genetically-engineered Escherichia-coli bacteria. Biopolymers 34, 1049-1058. 

Figure 3 Schematic illustration of a hybrid hydrogel system—genetically engineered coiled-coil protein domains used to crosslink synthetic water-soluble polymers. Divalent transition metal ions are shown to form complexes with nitrogen-oxygen-donor ligands on the synthetic polymer side chains and the terminal histidine residues in the coiled coils.

In succeeding sections we will consider the different types of biopolymer, and how their properties reflect their polymeric nature. The major groups of biopolymers are proteins, polysaccharides and nucleic acids. The latter are not to be considered as polymer materials in the present context, although they are the source of genetically engineered materials. Several other green polymers... 

Chilkoti A, Chen G, Stayton PS, Hoffman AS. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Bioconj Chem 1994 5 504-507. 

All strategies for the production of genetically engineered polymers rely on the basic principle of self-figation (concata-merization) of DNA monomers to form concatameric DNA sequences (3,4). These concatamers are then cloned into the appropriate plasmid and introduced into a biological expression system, where they are transcribed and translated by the ceUrdar machinery to produce a protein-based pol3nner. 

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