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Protein/peptide analysis size-exclusion chromatography

Detection in 2DLC is the same as encountered in one-dimensional HPLC. A variety of detectors are presented in Table 5.2. The choice of detector is dependent on the molecule being detected, the problem being solved, and the separation mode used for the second dimension. If MS detection is utilized, then volatile buffers are typically used in the second-dimension separation. Ultraviolet detection is used for peptides, proteins, and any molecules that contain an appropriate chromophore. Evaporative light scattering detection has become popular for the analysis of polymers and surfactants that do not contain UV chromophores. Refractive index (RI) detection is generally used with size exclusion chromatography for the analysis of polymers. [Pg.109]

Schmidt A-C, Fahlbusch B, Otto M. Size exclusion chromatography coupled to electrospray ionization mass spectrometry for analysis and quantitative characterization of arsenic interactions with peptides and proteins. J Mass Spectrom 2009 44 898-910. [Pg.223]

Oven/iew Waters, Sediments, and Soils. Ion-Selective Electrodes Water Applications. Isotope Dilution Analysis. Liquid Chromatography Size-Exclusion Liquid Chromatography-Mass Spectrometry Mass Spectrometry Peptides and Proteins. Voltammetry Overview. [Pg.332]

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See also in sourсe #XX -- [ Pg.839 ]



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Peptides chromatography

Peptides size-exclusion chromatography

Protein analysis

Protein/peptide analysis chromatography

Proteins chromatography

Size analysis

Size chromatography

Size-exclusion

Sizings, analysis

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