Protein mimetic imprinted gels responsive hydrogels exhibiting biomolecular recognition properties

PROTEIN MIMETIC IMPRINTED GELS RESPONSIVE HYDROGELS EXHIBITING BIOMOLECULAR RECOGNITION PROPERTIES 

Bio-imprinting polymeric receptors with and for biological macromolecules 

In a subsequent study, the Co coordinated assembly was grafted onto a cross-linked poly(glycidoxypropylmethacrylate) (GMA)-ethylene glycol dimethacrylate (EDMA) support surface. The template and Co ions were then leached out from the matrix. This surface grafted polymer exhibited turnover, Michaelis-Menten kinetics and mechanisms such as inhibition by tosyl phenyl alanyl chloromethyl ketone and inhibition on acetylation of hydroxyl group of 2-hydroxyethylmethacry-late (HEMA), similar to native chymotrypsin. 

It was earlier reported that copolymerisation of HEMA, MAA and methacrylate-histidine coordinated with Co resulted in a copolymer in which the three monomers are so placed along the polymer chain that they could be again brought close to each other to form a complex with Co. Due to the different monomer reactivity ratios of the three monomers, such placement of monomers was not observed when copolymerisation was carried out in the absence of Co. This suggests that monomers bearing hydroxyl, carboxyl and imidazole groups, when coordinated with Co, undergo polymerisation as a monomeric Co(II) complex and do not form a random copolymer of the three monomers. 

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