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Protein folding automated analysis

Some years ago, in an effort to improve previous approaches (3-9), we developed an HPLC-derived method to automate analysis of protein folding and preparative... [Pg.459]

Matrix effects experienced in the analysis of microsomal incubation products (Ch. 10.6.1) were evaluated by Zheng et al. [92]. The individual effects of the Tris buffer, NADPH, and the microsomes on the ESI-MS response of 27 different drags were investigated in direct injection MS-MS experiments. The more polar analytes showed up to 5-fold ion suppression. Therefore, an automated Oasis-HLB SPE procedure was developed in 96-well plate format. Direct injection of protein-precipitated incubations yielded similar results. Additional use of fast LC separation prior to MS-MS analysis gave no further improvement. [Pg.311]

See other pages where Protein folding automated analysis is mentioned: [Pg.96]    [Pg.75]    [Pg.459]    [Pg.465]    [Pg.466]    [Pg.275]    [Pg.529]    [Pg.169]    [Pg.166]    [Pg.276]    [Pg.338]    [Pg.143]    [Pg.391]    [Pg.82]    [Pg.460]    [Pg.214]    [Pg.256]    [Pg.512]    [Pg.357]    [Pg.358]    [Pg.176]    [Pg.361]    [Pg.153]    [Pg.386]    [Pg.550]   
See also in sourсe #XX -- [ Pg.459 , Pg.460 , Pg.461 , Pg.462 , Pg.463 , Pg.464 , Pg.465 ]



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