Protein Engineering to Improve Enzymatic Activity and Alter Substrate Specificity

3 PROTEIN ENGINEERING TO IMPROVE ENZYMATIC ACTIVITY AND ALTER SUBSTRATE SPECIFICITY 

Enzyme Organism Method Amino Acid Substitution(s) Feature Changes Reference 

Aniline dioxygenase Acinetobacter sp. strain YAA Saturation mutagenesis and error-prone PCR V205A/I248L/S404C Increased activity Widened substrate specificity to include 2,4-dimethylaniline and 2-isopropylaniline [20] 

Biphenyl dioxygenase B. xenovorans LB400 Family shuffling of bphA from polychlorinated biphenyl-contaminated soil DNA Multiple mutations (variant SlOO) Attack on carbon 5 and 6 of 2,2 -dichlorobiphenyl new ability to oxidize benzene, ethylbenzene, and toluene [22] 

Biphenyl dioxygenase P. pseudoalcaligenes KF707 Site-directed mutagenesis T376A/V/F/L T376F Novel ability to oxidize dibenzofuran Enhanced (KF707 enzyme) activities toward various polychlorinated biphenyls [24] 

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