Protein Engineering for Biocatalytic Epoxidation Reaction

Protein engineering, a design or optimization process of proteins, has become a powerful tool in biotechnology to generate engineered biocatalysts to meet the production requirements of pharmaceuticals, biofuels, and other chemicals [124-128]. 

Taking advantage of the development of efficient screening strategies and elucidation of crystal structures, both directed evolution and rational design approaches have been successfully used to engineer enzymes involved in bioepoxidation for improved activity, stereoselectivity, or protein stability, or modified substrate specificity (Table 13.1). 

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