Protein chromatography conventional packings

Summarizing the considerations on particle side mass transport, slow protein diffusion in porous adsorbents seems to have the same dominant influence on the efficiency of a fluidized bed adsorption as it frequently is the case in a packed bed. Contrary to conventional protein chromatography increasing... 

The performance of a conventional method of protein purification may generally be characterized by its capacity and resolution, in the case of a primary recovery operation the additional criteria of clarification efficiency and reduction of process volume become important parameters describing a successful procedure. In packed bed chromatography of proteins on porous adsorbents there are four main system parameters influencing the overall performance ... 

As the ligand-protein interaction takes place at the internal surface of porous adsorbents, kinetics and equilibrium of the interaction should be independent of the interstitial voidage within an adsorbent bed. Therefore the equilibrium capacity of an adsorbent will not be influenced by different experimental configurations e.g. batch stirred tank, batch fluidized bed, frontal application to packed or fluidized beds. The major difference arises from the medium from which the protein is isolated. As fluidized beds are used for whole broth adsorption, the properties of the broth have to be considered regarding the possible influence of components which are removed in conventional primary recovery steps and therefore are not present during the initial chromatography operations in a standard downstream process. These are on one hand nucleic... 

Ampholyte displacement chromatography [43] does not require special packings to be applied. Conventional ion exchangers are sufficient. The main principle is the elution of the column with carrier ampholytes. Leaback and Robinson [43] used CM-cellulose for separation of acetyl hexosamidase isoenzymes (Fig. 4.5.3), Young and Webb [44,50] separated serum proteins on DEAE-cellulose, and Chapuis-Cellier et al. [97] described ampholyte displacement chromatography of hemoglobins on DEAE-cellulose. 

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