3- propionic acid, hydrogen peroxide determination

Hydroxyphenyl)propionic acid, hydrogen peroxide determination, 639 6-Hydroxy-l,2,4-trioxanes, synthesis, 288 Hypercholesterolemia, reactive oxygen species in blood, 612... 

Roulier et al. reported a sensitive and specific method for the measurement of choline and hydrogen peroxide in sea-water [45]. Choline was oxidized by choline oxidase to produce betaine and H202. The latter was used with horse-radish peroxidase to oxidize hydro-xyphenyl-propionic acid to produce a fluorescent diphenol end product. The resulting fluorescence at 410 nm (excitation at 320 nm) was proportional to the amount of H202, and could thus be used to measure the amount of choline present in the sample. Only 2-dimethyl aminoethanol interfered. The method was optimized, and used to determine 0 15 nM choline in coastal sea-water. 

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