Promoter site digestion

The first step in creating a specific mRNA species is to construct a plasmid encoding the mRNA behind a T7, T3, or SP6 RNA polymerase promoter sequence. The S boundary of the mRNA is defined by the position of the promoter transcription start site and the S end is defined by restriction digest of the plasmid prior to in vitro transcription. 

It is essential to the theme of this chapter that quality interpretation shapes visitors on-site experiences and helps promote sustainable behaviour. There is only limited evidence for this claim, and more work needs to be carried out more creatively in this area. In a number of individual studies learning and educational efforts as well as enjoyment have been demonstrated as mutual outcomes of interpretive efforts (Moscardo, 1998). Additionally, interpretation can clearly structure a visitor s time at a site, both in the activity itself and in the ways in which the material digested shapes further on-site activity. It is less clear that tourist-related on-site interpretation fosters long-term behavioural changes that serve the overall conservation of the planet. Nevertheless for select groups predisposed to certain kinds of tourism, existing attitudes, appear to be consolidated (Lee, 2002). 

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