Progesterone binding

The next publication appeared in 1984. Venton et al. [67] entrapped antiprogesterone antiserum into a sol-gel derived silica. It retained about 56 % of progesterone binding capacity. The authors mentioned the considerable promise of the sol-gel technique for biopolymer immobilization, but their study was not further continued. 

The excess of the activated receptor, at least in the case of the progesterone, binds with acid proteins that function like acceptors. Two possible functions are attributed to these proteins that have not been confirmed that of being an active receptor reservoir and that of being responsible for directing the excess of the receptor toward degradation (Filardo 2002 Gruber et al. 2002). 

B. RU 486 acts as a competitive progesterone antagonist and blocks progesterone binding at its receptors. It has no activity at estrogenic receptors. 

Albani, J.R. (2006) Progesterone binding to the tryptophan residues of human ai-acid glycoprotein. 

Kute, T. and Westphal, U. (1976) Steroid-protein interactions. XXXIV. Chemical modification of a 1 -acid glycoprotein for characterization of the progesterone binding site. Biochimica el Biophysica Acta 420, 195-213. 

AR/ERa/progesterone -binding assays Binding and induction/inhibition of reporter gene product Mechanistic studies... 

Pearlman WH, Gueriguian JL, Sawyer ME. A specific progesterone-binding component of human breast cyst fluid. J Biol Chem. 1973 248 5736-5741. 

Fig. 2.1 (c) Stereo view of the peripheral progesterone-binding site in CYP3A4. The solvent-accessible surface of CYP3A4 is depicted as a semitransparent surface in blue, while the solvent-... 

Sakamoto, H., Ukena, K., Kawata, M., and Tsutsui, K. (2007). Expression, localization and possible actions of 25-Dx, a membrane-associated putative progesterone-binding protein, in the developing Purkinje cell of the cerebellum A new insight into the biosynthesis, metabolism and multiple actions of progesterone as a neurosteroid. Cerebellum Mar 2 1-8 [Epub ahead of print]. 

Progesterone binds to a i-acid glycoprotein on a hydrophobic region of a pocket present in the protein. Figure 1.35 shows the absorption spectrum of 13 pM ai-acid glycoprotein in buffer at pH 7, its first and second derivative spectra... 

Wilson EA,Yang F et al (1980) Estradiol and progesterone binding in uterine leiomyomata and in normal uterine tissues. Obstet Gynecol 55 20-24... 

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