Profiling of Caco-2 Cells

Clearly, genome-wide studies contribute to a better understanding why differences in absorption along the intestine, and between in vitro and in vivo situations, can be expected. These studies could on the one hand confirm that the most widely used in vitro system to study drug absorption, namely, the Caco-2 cells, may not always be very accurate in predicting uptake rates for a new compound. On the other hand, it has to be noted that compared to other in vitro systems, which are being used to study transport in liver and kidney, the Caco-2 cells correlate certainly the best with the in vivo system with respect to the mRNA expression of transporters [3], 

From a medical chemistry point of view, genome-wide expression studies can be exploited to design drugs in such a way that highly expressed transporter proteins can 

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